› 8sgn

X-ray diffraction
2.2Å resolution

Crystal structure of Epstein-Barr virus glycoprotein 350 (gp350) in complex with Cy651H02, a monoclonal antibody isolated from macaques immunized with a gp350 nanoparticle vaccine

Released:
DOI: 10.2210/pdb8sgn/pdb
PDB entry 8sgn coloured by chain, front view.
PDB entry 8sgn coloured by chain, side view.
PDB entry 8sgn coloured by chain, top view.
Source organisms:
Entry authors: Joyce MG, Jensen JL, Chen WH, Kanekiyo M

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-243124 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Cy651H02 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 22.49 KDa
Source organism: Macaca fascicularis
Expression system: Homo sapiens
Cy651H02 Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 223 amino acids
Theoretical weight: 23.77 KDa
Source organism: Macaca fascicularis
Expression system: Homo sapiens
Envelope glycoprotein GP350 Chain: G
Molecule details ›
Chain: G
Length: 431 amino acids
Theoretical weight: 46.9 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Homo sapiens
UniProt:
  • Canonical: P03200 (Residues: 1-425; Coverage: 47%)
Gene name: BLLF1
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
3 bound ligands:
Ligand EDO 23 x EDO
Ligand TRS 4 x TRS
Ligand NAG 9 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: R3
Unit cell:
a: 139.148Å b: 139.148Å c: 172.337Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.193 0.237
Expression system: Homo sapiens

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