› 8si0

X-ray diffraction
2.7Å resolution

Structure of binary complex of human cGAS and bound cGAMP

Released:
DOI: 10.2210/pdb8si0/pdb
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PDB entry 8si0 coloured by chain, front view.
PDB entry 8si0 coloured by chain, side view.
PDB entry 8si0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structural basis for 2'-5'/3'-5'-cGAMP synthesis by cGAS.
Wu S, Gabelli SB, Sohn J
OpenAccess logo Nat Commun 15 4012 (2024)
PMID: 38740774

Function and Biology Details

Reaction catalysed: 
(1a) ATP + GTP = pppG-P(2'-5')A + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic GMP-AMP synthase Chain: A
Molecule details ›
Chain: A
Length: 369 amino acids
Theoretical weight: 42.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8N884 (Residues: 157-522; Coverage: 70%)
Gene names: C6orf150, CGAS, MB21D1
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand 1SY 1 x 1SY
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS-II BEAMLINE 17-ID-2
Unit cell:
a: 47.927Å b: 59.657Å c: 125.327Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.217 0.28
Expression system: Escherichia coli BL21(DE3)

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