› 8sif

X-ray diffraction
1.7Å resolution

Crystal structure of Escherichia coli HPPK in complex with bisubstrate inhibitor HP-101

Released:
DOI: 10.2210/pdb8sif/pdb
PDB entry 8sif coloured by chain, front view.
PDB entry 8sif coloured by chain, side view.
PDB entry 8sif coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Shaw GX, Shi G, Ji X

Function and Biology Details

Reaction catalysed: 
ATP + 6-hydroxymethyl-7,8-dihydropterin = AMP + 6-hydroxymethyl-7,8-dihydropterin diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150686 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 18.1 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P26281 (Residues: 1-159; Coverage: 100%)
Gene names: JW0138, b0142, folK
Sequence domains: 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase (HPPK)

Ligands and Environments

1 bound ligand:
Ligand ZX4 1 x ZX4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 80.625Å b: 52.576Å c: 36.512Å
α: 90° β: 102.58° γ: 90°
R-values:
R R work R free
0.181 0.178 0.235
Expression system: Escherichia coli

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