› 8sjd

Electron Microscopy
5.1Å resolution

Cryo-EM structure of the Hermes transposase bound to two right-ends of its DNA transposon.

Released:
DOI: 10.2210/pdb8sjd/pdb
PDB entry 8sjd coloured by chain, front view.
PDB entry 8sjd coloured by chain, side view.
PDB entry 8sjd coloured by chain, top view.
Source organism: Musca domestica
Primary publication:
Zinc-finger BED domains drive the formation of the active Hermes transpososome by asymmetric DNA binding.
Lannes L, Furman CM, Hickman AB, Dyda F
OpenAccess logo Nat Commun 14 4470 (2023)
PMID: 37491363
Related structures: EMD-40553

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-222939 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
BED-type domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 612 amino acids
Theoretical weight: 70.21 KDa
Source organism: Musca domestica
Expression system: Escherichia coli
UniProt:
  • Canonical: Q25438 (Residues: 1-612; Coverage: 100%)
Sequence domains:
DNA (55-MER) Chains: G, J
Molecule details ›
Chains: G, J
Length: 55 nucleotides
Theoretical weight: 16.91 KDa
Source organism: Musca domestica
Expression system: Not provided
DNA (46-MER) Chains: F, I
Molecule details ›
Chains: F, I
Length: 46 nucleotides
Theoretical weight: 14.2 KDa
Source organism: Musca domestica
Expression system: Not provided
DNA (8-MER) Chains: E, H
Molecule details ›
Chains: E, H
Length: 7 nucleotides
Theoretical weight: 2.16 KDa
Source organism: Musca domestica
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.1Å
Relevant EMDB volumes: EMD-40553
Expression systems:
  • Escherichia coli
  • Not provided

Quick links