› 8sjg

X-ray diffraction
2.39Å resolution

Zn-Bound Structure of Computationally Designed Homotrimer Tet4

Released:
DOI: 10.2210/pdb8sjg/pdb
PDB entry 8sjg coloured by chain, front view.
PDB entry 8sjg coloured by chain, side view.
PDB entry 8sjg coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Atomically Accurate Design of Metalloproteins with Predefined Coordination Geometries.
Hoffnagle AM, Tezcan FA
J Am Chem Soc (2023)
PMID: 37352018

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-220151 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tet4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 114 amino acids
Theoretical weight: 12.56 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: R3
Unit cell:
a: 67.663Å b: 67.663Å c: 109.635Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.245 0.24 0.286
Expression system: Escherichia coli BL21(DE3)

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Citations

1 mention without citation

Atomically Accurate Design of Metalloproteins with Predefined Coordination Geometries.
Hoffnagle et al. (2023)