› 8sju

X-ray diffraction
7.14Å resolution

[4T17] Self-assembling right-handed four-turn tensegrity triangle with 17 interjunction base pairs and R3 symmetry

Released:
DOI: 10.2210/pdb8sju/pdb
PDB entry 8sju coloured by chain, front view.
PDB entry 8sju coloured by chain, side view.
PDB entry 8sju coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Engineering tertiary chirality in helical biopolymers.
Janowski J, Pham VAB, Vecchioni S, Woloszyn K, Lu B, Zou Y, Erkalo B, Perren L, Rueb J, Madnick J, Mao C, Saito M, Ohayon YP, Jonoska N, Sha R
Proc Natl Acad Sci U S A 121 e2321992121 (2024)
PMID: 38684000

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-243383 (preferred)
Entry contents:
4 distinct DNA molecules
Macromolecules (4 distinct):
DNA (5'-D(*GP*AP*AP*AP*AP*AP*CP*AP*CP*TP*GP*CP*CP*TP*GP*AP*AP*TP*AP*CP*CP*GP*CP*A)-3') Chain: A
Molecule details ›
Chain: A
Length: 24 nucleotides
Theoretical weight: 7.34 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*GP*CP*GP*GP*TP*AP*TP*TP*CP*AP*CP*CP*AP*CP*GP*AP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 17 nucleotides
Theoretical weight: 5.19 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (25-MER) Chain: C
Molecule details ›
Chain: C
Length: 25 nucleotides
Theoretical weight: 7.72 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*TP*CP*GP*TP*GP*GP*AP*CP*TP*GP*AP*CP*TP*CP*AP*AP*AP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 18 nucleotides
Theoretical weight: 5.52 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: R3
Unit cell:
a: 224.532Å b: 224.532Å c: 126.522Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.113 0.111 0.177
Expression system: Not provided

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