› 8smk

Electron Microscopy
3.5Å resolution

hPAD4 bound to Activating Fab hA362

Released:
DOI: 10.2210/pdb8smk/pdb
PDB entry 8smk coloured by chain, front view.
PDB entry 8smk coloured by chain, side view.
PDB entry 8smk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Antibody discovery identifies regulatory mechanisms of protein arginine deiminase 4.
Zhou X, Kong S, Maker A, Remesh SG, Leung KK, Verba KA, Wells JA
OpenAccess logo Nat Chem Biol (2024)
PMID: 38308046
Related structures: EMD-40589

Function and Biology Details

Reaction catalysed: 
Protein L-arginine + H(2)O = protein L-citrulline + NH(3)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-240944 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein-arginine deiminase type-4 Chains: A, D
Molecule details ›
Chains: A, D
Length: 695 amino acids
Theoretical weight: 77.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UM07 (Residues: 2-663; Coverage: 100%)
Gene names: PAD4, PADI4, PADI5, PDI5
Sequence domains:
Activating Fab 362 heavy chain Chains: B, E
Molecule details ›
Chains: B, E
Length: 229 amino acids
Theoretical weight: 24.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Activating Fab 362 light chain Chains: C, F
Molecule details ›
Chains: C, F
Length: 215 amino acids
Theoretical weight: 23.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand CA 8 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-40589
Expression system: Escherichia coli

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