› 8smr

Electron Microscopy
2.7Å resolution

cytochrome bc1-cbb3 supercomplex from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb8smr/pdb
PDB entry 8smr coloured by chain, front view.
PDB entry 8smr coloured by chain, side view.
PDB entry 8smr coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structure of the bc1-cbb3 respiratory supercomplex from Pseudomonas aeruginosa.
Di Trani JM, Gheorghita AA, Turner M, Brzezinski P, Ädelroth P, Vahidi S, Howell PL, Rubinstein JL
Proc Natl Acad Sci U S A 120 e2307093120 (2023)
PMID: 37751552
Related structures: EMD-40601

Function and Biology Details

Reactions catalysed: 
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
4 ferrocytochrome c + O(2) + 8 H(+)(Side 1) = 4 ferricytochrome c + 4 H(2)O(Side 2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
PDBe Complex ID:
PDB-CPX-523291 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Ubiquinol-cytochrome c reductase iron-sulfur subunit Chains: C, Z
Molecule details ›
Chains: C, Z
Length: 194 amino acids
Theoretical weight: 20.56 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: Q9HVY4 (Residues: 3-196; Coverage: 99%)
Gene name: PA4431
Sequence domains:
Cytochrome b Chains: D, I
Molecule details ›
Chains: D, I
Length: 403 amino acids
Theoretical weight: 46.11 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: Q9HVY5 (Residues: 1-403; Coverage: 100%)
Gene name: PA4430
Sequence domains:
Probable cytochrome c1 Chains: J, M
Molecule details ›
Chains: J, M
Length: 233 amino acids
Theoretical weight: 26.28 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: Q9HVY6 (Residues: 25-257; Coverage: 97%)
Gene name: PA4429
Sequence domains: Cytochrome C1 family
Cytochrome c4 Chains: K, N
Molecule details ›
Chains: K, N
Length: 181 amino acids
Theoretical weight: 18.7 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: P00106 (Residues: 21-201; Coverage: 100%)
Gene names: PA5490, cc4
Sequence domains: Cytochrome c
Cytochrome c domain-containing protein Chains: L, O
Molecule details ›
Chains: L, O
Length: 136 amino acids
Theoretical weight: 13.48 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: Q9HTQ4 (Residues: 1-136; Coverage: 100%)
Gene names: PA5300, cycB
Sequence domains: Cytochrome C oxidase, cbb3-type, subunit III
cytochrome-c oxidase Chain: E
Molecule details ›
Chain: E
Length: 468 amino acids
Theoretical weight: 52.59 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: Q9HWP8 (Residues: 8-475; Coverage: 99%)
Gene name: PA4133
Sequence domains: Cytochrome C and Quinol oxidase polypeptide I
Cytochrome c domain-containing protein Chain: F
Molecule details ›
Chain: F
Length: 200 amino acids
Theoretical weight: 22.42 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: Q9I3G4 (Residues: 1-200; Coverage: 99%)
Gene names: PA1553, ccoO1
Sequence domains: Cytochrome C oxidase, mono-heme subunit/FixO
Cbb3-type cytochrome c oxidase subunit Chain: G
Molecule details ›
Chain: G
Length: 312 amino acids
Theoretical weight: 34.08 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: Q9I3G5 (Residues: 1-312; Coverage: 98%)
Gene names: PA1552, ccoP1
Sequence domains:

Ligands and Environments

7 bound ligands:
Ligand FES 2 x FES
Ligand HEM 6 x HEM
Ligand U10 2 x U10
Ligand I7Y 2 x I7Y
Ligand HEC 9 x HEC
Ligand CU 1 x CU
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-40601

Quick links

Citations

3 citation in other articles

Structure of the bc1-cbb3 respiratory supercomplex from Pseudomonas aeruginosa.
Di Trani et al. (2023)
2 more