› 8smu

X-ray diffraction
2.45Å resolution

Integral fusion of the HtaA CR2 domain from Corynebacterium diphtheriae within EGFP

Released:
DOI: 10.2210/pdb8smu/pdb
PDB entry 8smu coloured by chain, front view.
PDB entry 8smu coloured by chain, side view.
PDB entry 8smu coloured by chain, top view.
Source organisms:
Primary publication:
Development and atomic structure of a new fluorescence-based sensor to probe heme transfer in bacterial pathogens.
Mahoney BJ, Goring AK, Wang Y, Dasika P, Zhou A, Grossbard E, Cascio D, Loo JA, Clubb RT
J Inorg Biochem 249 112368 (2023)
PMID: 37729854

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229954 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein; Htaa domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 403 amino acids
Theoretical weight: 44 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6NIZ1 (Residues: 344-507; Coverage: 29%)
  • Canonical: P42212 (Residues: 40-238; Coverage: 84%)
Gene names: DIP0625, GFP
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand HEM 4 x HEM
Ligand GOL 23 x GOL
Ligand 1PE 4 x 1PE
Ligand CL 4 x CL
1 modified residue:
Ligand CRO 4 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 63.826Å b: 272.783Å c: 73.518Å
α: 90° β: 114.22° γ: 90°
R-values:
R R work R free
0.21 0.209 0.233
Expression system: Escherichia coli BL21(DE3)

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