› 8smv

Electron Microscopy
2.74Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-240254 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
G-protein coupled receptor 161 Chain: R
Molecule details ›
Chain: R
Length: 540 amino acids
Theoretical weight: 59.87 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8N6U8 (Residues: 1-529; Coverage: 100%)
Gene name: GPR161
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 142 amino acids
Theoretical weight: 15.4 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 30.14 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P63092 (Residues: 5-64, 204-394; Coverage: 61%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 370 amino acids
Theoretical weight: 40.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat

Ligands and Environments

1 bound ligand:
Ligand CLR 1 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.74Å
Relevant EMDB volumes: EMD-40603
Expression systems:
  • Homo sapiens
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

1 citations in other articles

Context-dependent ciliary regulation of hedgehog pathway repression in tissue morphogenesis.
Hwang et al. (2023)