› 8soj

Electron Microscopy
3.8Å resolution

Cryo-EM structure of human CST bound to POT1(ESDL)/TPP1 in the absence of telomeric ssDNA

Released:
DOI: 10.2210/pdb8soj/pdb
PDB entry 8soj coloured by chain, front view.
PDB entry 8soj coloured by chain, side view.
PDB entry 8soj coloured by chain, top view.
Source organisms:
Primary publication:
POT1 recruits and regulates CST-Polα/primase at human telomeres.
Cai SW, Takai H, Zaug AJ, Dilgen TC, Cech TR, Walz T, de Lange T
Cell 187 3638-3651.e18 (2024)
PMID: 38838667
Related structures: EMD-40659

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-231597 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
CST complex subunit CTC1; Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 1613 amino acids
Theoretical weight: 178.3 KDa
Source organisms: Expression system: Trichoplusia ni
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: Q2NKJ3 (Residues: 1-1217; Coverage: 100%)
Gene names: C17orf68, CTC1, JW3994, b4034, malE
Sequence domains:
Adrenocortical dysplasia protein homolog; CST complex subunit STN1 Chain: B
Molecule details ›
Chain: B
Length: 1049 amino acids
Theoretical weight: 115.63 KDa
Source organisms: Expression system: Trichoplusia ni
UniProt:
  • Canonical: B6F2F5 (Residues: 2-241; Coverage: 10%)
  • Canonical: Q96AP0 (Residues: 1-397; Coverage: 87%)
  • Canonical: Q9H668 (Residues: 1-368; Coverage: 100%)
Gene names: ACD, OBFC1, PIP1, PTOP, STN1, TINT1, TPP1
Sequence domains:
CST complex subunit TEN1 Chain: C
Molecule details ›
Chain: C
Length: 123 amino acids
Theoretical weight: 13.87 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q86WV5 (Residues: 1-123; Coverage: 100%)
Gene names: C17orf106, TEN1
Sequence domains: Telomere-capping, CST complex subunit
Protection of telomeres protein 1 Chain: D
Molecule details ›
Chain: D
Length: 646 amino acids
Theoretical weight: 72.94 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9NUX5 (Residues: 2-320, 321-634; Coverage: 100%)
Gene name: POT1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-40659
Expression system: Trichoplusia ni

Quick links

Citations

1 citations in other articles

Shieldin and CST co-orchestrate DNA polymerase-dependent tailed-end joining reactions independently of 53BP1-governed repair pathway choice.
King et al. (2024)

1 mention without citation

POT1 recruits and regulates CST-Polα/Primase at human telomeres.
Cai et al. (2023)