› 8spe

X-ray diffraction
2.3Å resolution

Crystal structure of Bax core domain BH3-groove dimer - tetrameric fraction P31

Released:
DOI: 10.2210/pdb8spe/pdb
Model geometry
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PDB entry 8spe coloured by chain, front view.
PDB entry 8spe coloured by chain, side view.
PDB entry 8spe coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Sequence differences between BAX and BAK core domains manifest as differences in their interactions with lipids.
Miller MS, Cowan AD, Brouwer JM, Smyth ST, Peng L, Wardak AZ, Uren RT, Luo C, Roy MJ, Shah S, Tan Z, Reid GE, Colman PM, Czabotar PE
FEBS J (2023)
PMID: 38088212

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274476 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Apoptosis regulator BAX Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c, d, e, f, g, h, i, j
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c, d, e, f, g, h, i, j
Length: 81 amino acids
Theoretical weight: 8.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07812 (Residues: 53-128; Coverage: 40%)
  • Best match: Q07812-5 (Residues: 53-124)
Gene names: BAX, BCL2L4
Sequence domains: Apoptosis regulator proteins, Bcl-2 family

Ligands and Environments

3 bound ligands:
Ligand ZN 9 x ZN
Ligand EDO 6 x EDO
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P31
Unit cell:
a: 141.355Å b: 141.355Å c: 110.191Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.229 0.256
Expression system: Escherichia coli

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