8spq

Electron Microscopy
3.26Å resolution

SpRY-Cas9:gRNA complex targeting TGG PAM DNA

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-243669 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: A
Molecule details ›
Chain: A
Length: 1369 amino acids
Theoretical weight: 159.15 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99ZW2 (Residues: 2-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
TS Chain: C
Molecule details ›
Chain: C
Length: 55 nucleotides
Theoretical weight: 16.87 KDa
Source organism: Escherichia phage Lambda
Expression system: Not provided
NTS Chain: D
Molecule details ›
Chain: D
Length: 19 nucleotides
Theoretical weight: 5.85 KDa
Source organism: Escherichia phage Lambda
Expression system: Not provided
TS Chain: E
Molecule details ›
Chain: E
Length: 19 nucleotides
Theoretical weight: 5.76 KDa
Source organism: Escherichia phage Lambda
Expression system: Not provided
gRNA Chain: B
Molecule details ›
Chain: B
Length: 98 nucleotides
Theoretical weight: 31.7 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.26Å
Relevant EMDB volumes: EMD-40681
Expression systems:
  • Escherichia coli
  • Not provided