› 8sqa

Electron Microscopy
4.2Å resolution

The cryo-EM structure of the EcBAM/EspP(beta8-12) complex

Released:
DOI: 10.2210/pdb8sqa/pdb
PDB entry 8sqa coloured by chain, front view.
PDB entry 8sqa coloured by chain, side view.
PDB entry 8sqa coloured by chain, top view.
Source organisms:
Entry authors: Wu R, Noinaj N
Related structures: EMD-40700

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-128463 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Outer membrane protein assembly factor BamA Chain: A
Molecule details ›
Chain: A
Length: 786 amino acids
Theoretical weight: 88.1 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A940 (Residues: 25-810; Coverage: 100%)
Gene names: JW0172, b0177, bamA, yaeT, yzzN, yzzY
Sequence domains:
Outer membrane protein assembly factor BamB Chain: B
Molecule details ›
Chain: B
Length: 372 amino acids
Theoretical weight: 39.78 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P77774 (Residues: 21-392; Coverage: 100%)
Gene names: JW2496, b2512, bamB, yfgL
Sequence domains:
Outer membrane protein assembly factor BamC Chain: C
Molecule details ›
Chain: C
Length: 319 amino acids
Theoretical weight: 34.3 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A903 (Residues: 26-344; Coverage: 100%)
Gene names: JW2462, b2477, bamC, dapX, nlpB
Sequence domains: NlpB/DapX lipoprotein
Outer membrane protein assembly factor BamD Chain: D
Molecule details ›
Chain: D
Length: 225 amino acids
Theoretical weight: 25.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AC02 (Residues: 21-245; Coverage: 100%)
Gene names: JW2577, b2595, bamD, yfiO
Sequence domains: Outer membrane lipoprotein
Outer membrane protein assembly factor BamE Chain: E
Molecule details ›
Chain: E
Length: 93 amino acids
Theoretical weight: 10.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A937 (Residues: 21-113; Coverage: 99%)
Gene names: JW2598, b2617, bamE, smpA
Sequence domains: SmpA / OmlA family
Autotransporter protein EspP translocator; Maltose/maltodextrin-binding periplasmic protein Chain: F
Molecule details ›
Chain: F
Length: 551 amino acids
Theoretical weight: 60.67 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 26-392; Coverage: 99%)
  • Canonical: O32591 (Residues: 1195-1300; Coverage: 9%)
Gene names: JW3994, b4034, espP, malE, pssA
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-40700
Expression system: Escherichia coli BL21(DE3)

Quick links