8sqb

Electron Microscopy
3.8Å resolution

The cryo-EM structure of the EcBAM/EspP(beta7-12) complex

Released:
Source organism: Escherichia coli
Entry authors: Wu R, Noinaj N
Related structures: EMD-40701

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-243947 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Outer membrane protein assembly factor BamA Chain: A
Molecule details ›
Chain: A
Length: 786 amino acids
Theoretical weight: 88.1 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C3TPJ2 (Residues: 25-810; Coverage: 100%)
Gene names: A2J79_001324, A5U30_000296, ABE91_003690, ABT96_000025, ACN81_28495, ACU57_18535, ACW72_001733, ATM34_002785, AW118_17900, AWP47_05630, B6R15_000220, B6R31_002460, BANRA_00651, BANRA_03348, BCB93_004068, BE932_24020, BER14_07740, BG944_001864, BGM66_002013, BGZ_00304, BGZ_01203, BJI68_15680, BK300_10935, BK383_20200, BKL28_001074, BMT50_03970, BRV02_001577, BTB68_000231, BTQ06_06530, BVE17_000752, BXT93_00750, BvCmsKKP061_01626, BvCmsNSP007_03755, C0P57_001486, C1Q91_003240, C2M16_10840, C2R31_000619, C3F40_12200, C719_003093, C7B02_09115, C9114_06985, C9160_13545, C9Z68_04825, CF22_003987, CIG67_16620, CLG78_001120, CQ842_02855, CQ842_07140, CR538_20600, CTR35_003026, CV628_000431, CV83915_01397, CWS33_09660, D3G36_16715, D4M65_00125, D4N09_06760, D9E49_02105, D9J61_11550, DAH27_18735, DD762_01135, DIV22_06310, DL738_01695, DL968_03380, DNQ45_10075, DNX30_02230, DS732_05815, DTL43_05880, DU321_03690, E0I52_12900, E2865_00172, E4K51_05750, E5H86_15850, E6D34_00950, EAI46_03605, ECs0179, EF082_10735, EIA08_06365, EIZ93_07885, EN85_001940, EPS76_09925, EPS97_01105, EWK56_14925, F7F11_02035, F7G01_02685, F7N46_12575, F9413_16900, F9461_04780, F9B07_03480, FEH53_10940, FGAF1022_38370, FGAF848_51120, FIJ20_13935, FJQ40_14175, FKO60_12300, FOI11_012800, FOI11_22880, FPI65_00965, FPS11_04890, FVB16_04485, FWK02_04400, FZU14_18300, G3V95_02420, G3W53_17975, G4A38_19380, G4A47_18815, G5603_21720, G6Z99_21860, GAI89_16125, GAJ12_05920, GKF66_15225, GNW61_11420, GNZ05_09785, GOP25_07210, GP711_09570, GQE86_00785, GQM04_14355, GQM21_02740, GQN24_12865, GQN34_16145, GRC73_19630, GRW05_07735, GTP92_20185, GUC01_17920, H0O72_10145, HEP30_016645, HEP34_000941, HHH44_003655, HI055_000768, HI084_000165, HIA71_05310, HID72_001957, HIE29_003111, HJ488_000501, HJQ60_000684, HJS37_001619, HKA49_001644, HLX92_18930, HLZ39_04785, HLZ50_06920, HMV95_04545, HND12_10395, HV109_19340, HV209_01580, HVV39_17670, HVW04_10185, HVW43_11715, HVW90_09545, HVY77_20925, I6H02_18690, IFC14_000025, IFN33_000025, IT029_001934, J0541_003907, J8F57_004096, JNP96_05075, KTF03_000358, NCTC10082_01374, NCTC10089_04067, NCTC10429_04185, NCTC10865_05056, NCTC11112_03444, NCTC11181_01353, NCTC13148_05206, NCTC8009_07311, NCTC8621_04193, NCTC8959_04852, NCTC8960_01553, NCTC9073_03384, NCTC9703_03426, NCTC9706_01328, OFN31_18745, OGM49_04200, PWL68_003278, QDW62_21630, R8G00_24975, R8O40_001034, RZR61_12560, SAMEA3472044_04148, SAMEA3752557_00883, TUM18780_35950, WR15_02370, bamA, yaeT
Sequence domains:
Outer membrane protein assembly factor BamB Chain: B
Molecule details ›
Chain: B
Length: 372 amino acids
Theoretical weight: 39.78 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P77774 (Residues: 21-392; Coverage: 100%)
Gene names: JW2496, b2512, bamB, yfgL
Sequence domains:
Outer membrane protein assembly factor BamC Chain: C
Molecule details ›
Chain: C
Length: 319 amino acids
Theoretical weight: 34.3 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C3T0D3 (Residues: 27-345; Coverage: 93%)
Gene names: ECs3339, bamC
Sequence domains: NlpB/DapX lipoprotein
Outer membrane protein assembly factor BamD Chain: D
Molecule details ›
Chain: D
Length: 225 amino acids
Theoretical weight: 25.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C3SYV7 (Residues: 21-245; Coverage: 100%)
Gene names: A2J79_005130, A5U30_004741, ABT96_004659, ACN81_10220, ACU57_00145, ACW72_004531, ATM34_004519, AWP47_02730, B6R15_004138, BANRA_01140, BANRA_03461, BCB93_004635, BE932_10795, BG944_004801, BGM66_003561, BGZ_03302, BGZ_03380, BJI68_05065, BK300_25530, BK383_10865, BMT50_17490, BRV02_004551, BTB68_003652, BTQ06_08815, BVE17_004293, BXT93_09590, BvCmsKKP061_02410, BvCmsNSP007_04548, C0P57_003558, C1Q91_004667, C2M16_27675, C2R31_001280, C3F40_23925, C4M78_00025, C719_003793, C7B02_26385, C9114_19725, C9160_23595, CF22_004780, CG704_21490, CIG67_05945, CLG78_004957, CQ842_19275, CQ842_21825, CR538_06370, CTR35_004171, CV628_003820, CWS33_25705, D3G36_23495, D4M65_08495, D9D43_23030, D9J61_06985, DAH27_25575, DD762_23835, DIV22_27340, DL968_25250, DNX30_24520, DS732_19135, DTL43_21715, DU321_27065, E0I52_20405, E2865_03649, E5H86_23160, E6D34_21890, EAI46_08680, ECs3458, EF082_15605, EIA08_26325, EIZ93_15605, EN85_004104, EPS76_07460, EPS97_18890, EWK56_06120, ExPECSC038_01360, F7G01_23410, F7N46_26030, F9461_09605, F9B07_09675, FEH53_24200, FGAF1022_12810, FGAF848_35140, FIJ20_24890, FJQ40_22945, FOI11_00020, FOI11_023160, FPI65_16265, FPS11_27775, FVB16_17575, G3V95_09835, G3W53_10590, G4A38_21100, G4A47_01215, G5603_26460, G6Z99_06280, GAI89_21195, GAJ12_00020, GKF66_26050, GNW61_26540, GNZ05_25675, GOP25_24440, GP711_05905, GP965_15905, GP975_17310, GQE86_18025, GQM04_13135, GQM21_13415, GQN24_22150, GQN34_14030, GRC73_20365, GRW05_07395, GRW24_26245, GTP92_23325, GUC01_18025, H0O72_19785, HEP30_021330, HEP34_004220, HHH44_004372, HI084_003730, HIA71_04105, HID72_005131, HIE29_001446, HJ488_005270, HJQ60_000005, HJS37_004772, HKA49_004919, HLX92_24210, HLZ50_23270, HMV95_03170, HND12_28045, HV109_05755, HV209_12625, HVW04_22055, HVW43_23355, HVW90_20030, HVY77_06825, I6H00_24740, I6H02_08585, IFC14_000799, IFN33_004713, IG021_002888, IH772_23730, IT029_001724, J0541_005120, J5U05_003358, J8F57_004930, JNP96_20765, KQO22_003439, KTF03_001083, NCTC10082_03688, NCTC10089_01252, NCTC10418_02013, NCTC10429_02002, NCTC10974_01477, NCTC11126_05258, NCTC11181_03444, NCTC11341_00985, NCTC7922_00800, NCTC7927_01375, NCTC7928_01472, NCTC8009_03231, NCTC8500_01327, NCTC8621_01335, NCTC8959_02527, NCTC8985_06064, NCTC9001_06581, NCTC9044_00364, NCTC9045_01469, NCTC9073_04650, NCTC9081_05672, NCTC9702_01502, NCTC9703_01233, NCTC9706_03511, NCTC9962_00805, OFN31_20485, OGM49_18310, P6223_005652, PWL68_001662, QDW62_06240, R8O40_004576, RZR61_19325, SAMEA3472044_03954, SAMEA3472112_00884, TUM18780_11060, WR15_13395, bamD, yfiO, yfiO_1
Sequence domains: Outer membrane lipoprotein
Outer membrane protein assembly factor BamE Chain: E
Molecule details ›
Chain: E
Length: 93 amino acids
Theoretical weight: 10.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A366UU94 (Residues: 21-113; Coverage: 100%)
Gene names: A2J79_004032, A5U30_004761, ABE91_013470, ABT96_004679, ACN81_10110, ACU57_00040, ACW72_004551, ATM34_004539, AW118_18475, AWP47_02615, B6R15_004158, B6R31_004759, BANRA_01160, BANRA_03480, BCB93_004656, BE932_10900, BER14_21305, BG944_004822, BGM66_003581, BGZ_03322, BGZ_03360, BJI68_04965, BK300_25630, BK383_10965, BKL28_002039, BMT50_17390, BRV02_004531, BTB68_003672, BTQ06_08460, BVE17_004314, BXT93_22155, BvCmsKKP061_02391, BvCmsNSP007_04567, C0P57_003578, C1Q91_004646, C2M16_24735, C2R31_001300, C3F40_23815, C4M78_00135, C719_003864, C7B02_26275, C9114_19830, C9160_23700, C9Z68_21235, CF22_004800, CG704_21595, CIG67_05840, CLG78_004560, CQ842_19380, CQ842_21925, CR538_06265, CTR35_004191, CV628_003840, CV83915_03496, D3C88_33860, D3G36_24940, D4M65_08385, D4N09_18960, D9D43_23135, D9E49_21045, D9J61_07090, DAH27_25685, DD762_23945, DIV22_27230, DL738_24845, DL968_07180, DNQ45_01360, DNX30_24630, DS732_19245, DTL43_21605, DU321_24480, E0I52_20295, E2865_03668, E4K51_10685, E5H86_23265, EAI46_08790, EF082_15710, EIA08_06590, EIZ93_15710, EN85_004125, EPS76_07565, EPS97_18785, F7F11_01805, F7G01_23510, F7N46_20005, F9413_21470, F9461_09705, F9B07_09575, FEH53_11990, FGAF1022_12620, FGAF848_35330, FIJ20_21810, FJQ40_23045, FOI11_00120, FOI11_023060, FPI65_16365, FPS11_21880, FVB16_18295, FWK02_09415, FZU14_20455, G3V95_09935, G3W53_10490, G4A38_21000, G4A47_01315, G5603_07570, G6Z99_06180, GAI89_21575, GAJ12_00120, GKF66_23890, GNW61_16365, GNZ05_25575, GOP25_24540, GP711_06005, GP954_12740, GP965_16485, GP975_20410, GQA06_18025, GQE86_17925, GQM04_26375, GQM13_06310, GQM21_13515, GQN24_22250, GQN34_14130, GRC73_20465, GRW05_07295, GRW24_17075, GTP92_23430, GUC01_18130, H0O72_19885, HEP30_021230, HEP34_004240, HHH44_004392, HI055_003787, HI084_003767, HIA71_04205, HID72_004034, HIE29_001466, HJ488_002391, HJQ60_000025, HJS37_004792, HKA49_004940, HLX92_22475, HLZ39_14830, HLZ50_20300, HMV95_03275, HND12_25520, HV109_05655, HV209_12725, HVV39_05080, HVW04_21950, HVW43_23255, HVW90_19930, HVY77_06725, I6H00_24640, I6H02_08695, IFN33_004693, IH772_08230, IT029_001744, J0541_004271, J5U05_003378, J8F57_004168, JNP96_20865, KQO22_003459, KTF03_001103, NCTC10082_03668, NCTC10089_01217, NCTC10418_01985, NCTC10429_01977, NCTC10764_00459, NCTC10767_02499, NCTC10865_01545, NCTC10974_01452, NCTC11112_07467, NCTC11126_05233, NCTC11181_03422, NCTC11341_01008, NCTC13148_02470, NCTC7922_00773, NCTC7927_01352, NCTC7928_01496, NCTC8009_02711, NCTC8179_06846, NCTC8500_01302, NCTC8621_00013, NCTC8621_01206, NCTC8622_03288, NCTC8959_02561, NCTC8960_03971, NCTC8985_06038, NCTC9044_00387, NCTC9045_01444, NCTC9073_04671, NCTC9075_01806, NCTC9077_01597, NCTC9081_05698, NCTC9117_01766, NCTC9702_01479, NCTC9703_05782, NCTC9706_03490, NCTC9962_00832, OFN31_20585, OGM49_18410, P6223_004692, QDW62_06140, R8G00_08025, R8O40_004596, RZR61_19225, SAMEA3472044_03974, SAMEA3472112_00955, TUM18780_10850, WR15_13295, bamE, smpA, smpA_1, smpA_2
Sequence domains: SmpA / OmlA family
Maltodextrin-binding protein Chain: F
Molecule details ›
Chain: F
Length: 581 amino acids
Theoretical weight: 64.02 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C3SHQ8 (Residues: 26-392; Coverage: 99%)
Gene names: ABE91_029440, ABT96_004252, ACN81_05700, ACU57_23670, ACW72_002521, ATM34_003353, B6R31_000964, BANRA_02708, BANRA_05111, BCB93_001091, BG944_002391, BGM66_004246, BGZ_01772, BGZ_04952, BJI68_06200, BK300_12795, BMT50_07545, BRV02_002252, BTB68_002078, BTQ06_17300, BVE17_001270, BvCmsKKP061_03224, BvCmsNSP007_02448, C0P57_003867, C1Q91_002164, C2R31_001890, C3F40_15210, C4M78_06410, C719_004008, C7B02_13920, C9160_08140, CF22_001770, CG704_16590, CIG67_12040, CLG78_001725, CQ842_10105, CQ842_11395, CR538_23895, CTR35_003815, CV628_004621, CV83915_02005, CWS33_15915, D4M65_12865, DAH27_04885, DIV22_28370, DL738_09430, DL968_20910, DNX30_07695, DS732_01860, DTL43_19585, E2865_05243, E4K51_08355, E5H86_20640, E6D34_15030, EAI46_20350, ECs5017, EIZ93_13775, EN85_000970, EPS97_17355, ExPECSC038_04540, F7G01_19710, F9461_21760, FEH53_22305, FGAF1022_44390, FGAF848_44030, FIJ20_18085, FJQ40_13885, FOI11_015465, FOI11_20215, FPS11_04610, FWK02_22115, G3V95_18070, G4A38_02205, G4A47_04495, G6Z99_18805, GAI89_05080, GAJ12_13200, GKF66_19285, GNW61_17855, GOP25_18965, GP965_07770, GP975_07695, GP979_10140, GQA06_09595, GQE86_14675, GQM04_22095, GQM21_08325, GQN24_10395, GRW05_14255, GRW24_12940, GUC01_08260, H0O72_20100, HEP30_015080, HHH44_003952, HIA71_09730, HID72_001572, HJ488_001404, HJS37_003418, HKA49_001043, HLX92_13085, HMV95_14740, HV109_22180, HV209_20940, HVW43_14700, HVW90_12355, HVY77_23840, I6H00_16895, I6H02_15990, IFN33_000590, IG021_004405, J0541_001933, J5U05_001620, J8F57_003138, JNP96_01525, KQO22_001235, KTF03_003565, NCTC10418_07064, NCTC10429_00012, NCTC10865_05806, NCTC11126_02082, NCTC11181_01902, NCTC13148_04480, NCTC8009_08341, NCTC8179_05034, NCTC8500_05253, NCTC8622_01707, NCTC8960_02276, NCTC8985_03950, NCTC9703_04157, NCTC9706_01951, NCTC9962_03706, OFN31_11500, P6223_003521, QDW62_24215, RZR61_19445, SAMEA3472044_04499, SAMEA3752557_02201, TUM18780_41180, WR15_07725, malE
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-40701
Expression system: Escherichia coli BL21(DE3)