› 8srs

Electron Microscopy
2.79Å resolution

SpRY-Cas9:gRNA complex targeting TAC PAM DNA

Released:
DOI: 10.2210/pdb8srs/pdb
PDB entry 8srs coloured by chain, front view.
PDB entry 8srs coloured by chain, side view.
PDB entry 8srs coloured by chain, top view.
Source organisms:
Primary publication:
Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9.
Hibshman GN, Bravo JPK, Hooper MM, Dangerfield TL, Zhang H, Finkelstein IJ, Johnson KA, Taylor DW
OpenAccess logo Nat Commun 15 3663 (2024)
PMID: 38688943
Related structures: EMD-40740

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-513189 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: A
Molecule details ›
Chain: A
Length: 1369 amino acids
Theoretical weight: 159.15 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99ZW2 (Residues: 2-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
TS Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 4 KDa
Source organism: Escherichia phage Lambda
Expression system: Not provided
NTS Chain: D
Molecule details ›
Chain: D
Length: 55 nucleotides
Theoretical weight: 16.97 KDa
Source organism: Escherichia phage Lambda
Expression system: Not provided
TS Chain: c
Molecule details ›
Chain: c
Length: 19 nucleotides
Theoretical weight: 5.76 KDa
Source organism: Escherichia phage Lambda
Expression system: Not provided
gRNA Chain: B
Molecule details ›
Chain: B
Length: 98 nucleotides
Theoretical weight: 31.7 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.79Å
Relevant EMDB volumes: EMD-40740
Expression systems:
  • Escherichia coli
  • Not provided

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