› 8ssz

Electron Microscopy
2.64Å resolution

The 2alpha3beta stoichiometry of full-length human alpha4beta2 nicotinic acetylcholine receptor in complex with acetylcholine and calcium

Released:
DOI: 10.2210/pdb8ssz/pdb
PDB entry 8ssz coloured by chain, front view.
PDB entry 8ssz coloured by chain, side view.
PDB entry 8ssz coloured by chain, top view.
Source organisms:
Primary publication:
Structural bases for stoichiometry-selective calcium potentiation of a neuronal nicotinic receptor.
Mazzaferro S, Kang G, Natarajan K, Hibbs RE, Sine SM
Br J Pharmacol 181 1973-1992 (2024)
PMID: 38454578
Related structures: EMD-40752

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-239668 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Neuronal acetylcholine receptor subunit alpha-4 Chains: A, D
Molecule details ›
Chains: A, D
Length: 601 amino acids
Theoretical weight: 67.41 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P43681 (Residues: 27-627; Coverage: 100%)
Gene names: CHRNA4, NACRA4
Sequence domains:
Neuronal acetylcholine receptor subunit beta-2 Chains: B, C, E
Molecule details ›
Chains: B, C, E
Length: 487 amino acids
Theoretical weight: 55.65 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P17787 (Residues: 26-502; Coverage: 100%)
Gene name: CHRNB2
Sequence domains:
IgG1 Kappa Light Chain Chains: F, H, J
IgG1 Heavy Chain Chains: G, I, K

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
5 bound ligands:
Ligand NAG 6 x NAG
Ligand ACH 2 x ACH
Ligand CA 2 x CA
Ligand NA 1 x NA
Ligand OCT 1 x OCT
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.64Å
Relevant EMDB volumes: EMD-40752
Expression system: Homo sapiens

Quick links

Citations

1 citations in other articles

Novel interplay between agonist and calcium binding sites modulates drug potentiation of α7 acetylcholine receptor.
Mukhtasimova et al. (2024)