› 8st4

Electron Microscopy
2.35Å resolution

The 2alpha3beta stoichiometry of human alpha4beta2 nicotinic acetylcholine receptor in complex with acetylcholine

Released:
DOI: 10.2210/pdb8st4/pdb
PDB entry 8st4 coloured by chain, front view.
PDB entry 8st4 coloured by chain, side view.
PDB entry 8st4 coloured by chain, top view.
Source organisms:
Primary publication:
Structural bases for stoichiometry-selective calcium potentiation of a neuronal nicotinic receptor.
Mazzaferro S, Kang G, Natarajan K, Hibbs RE, Sine SM
Br J Pharmacol 181 1973-1992 (2024)
PMID: 38454578
Related structures: EMD-40757

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-239671 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Neuronal acetylcholine receptor subunit alpha-4 Chains: A, D
Molecule details ›
Chains: A, D
Length: 386 amino acids
Theoretical weight: 44.86 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P43681 (Residues: 27-585, 586-627; Coverage: 64%)
Gene names: CHRNA4, NACRA4
Sequence domains:
Neuronal acetylcholine receptor subunit beta-2 Chains: B, C, E
Molecule details ›
Chains: B, C, E
Length: 403 amino acids
Theoretical weight: 46.75 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P17787 (Residues: 26-445, 446-502; Coverage: 82%)
Gene name: CHRNB2
Sequence domains:
IgG1 Kappa Light Chain Chains: F, H, J
Molecule details ›
Chains: F, H, J
Length: 238 amino acids
Theoretical weight: 26.38 KDa
Source organism: Mus musculus
IgG1 Heavy Chain Chains: G, I, K
Molecule details ›
Chains: G, I, K
Length: 462 amino acids
Theoretical weight: 51.2 KDa
Source organism: Mus musculus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
3 bound ligands:
Ligand NAG 6 x NAG
Ligand ACH 2 x ACH
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.35Å
Relevant EMDB volumes: EMD-40757
Expression system: Homo sapiens

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