› 8swk

Electron Microscopy
4.32Å resolution

Cryo-EM structure of NLRP3 closed hexamer

Released:
DOI: 10.2210/pdb8swk/pdb
PDB entry 8swk coloured by chain, front view.
PDB entry 8swk coloured by chain, side view.
PDB entry 8swk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structures of NLRP3 reveal its self-activation mechanism
Yu X, Matico RE, Miller R, Schoubroeck BV, Grauwen K, Suarez J, Pietrak B, Haloi N, Yin Y, Tresadern GJ, Perez-Benito L, Lindahl E, Bottelbergs A, Oehlrich D, Opdenbosch NV, Sharma S
Nat Commun (2024)
Related structures: EMD-40820

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188662 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NACHT, LRR and PYD domains-containing protein 3 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 910 amino acids
Theoretical weight: 103.68 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q96P20 (Residues: 133-1036; Coverage: 87%)
Gene names: C1orf7, CIAS1, NALP3, NLRP3, PYPAF1
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand 7YN 6 x 7YN
Ligand ATP 6 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.32Å
Relevant EMDB volumes: EMD-40820
Expression system: Spodoptera frugiperda

Quick links