› 8sxv

X-ray diffraction
2.3Å resolution

X-ray crystal structure of UDP- 2,3-diacetamido-2,3-dideoxy-glucuronic acid-2-epimerase from Thermus thermophilus strain HB27, apo form, pH 9

Released:
DOI: 10.2210/pdb8sxv/pdb
PDB entry 8sxv coloured by chain, front view.
PDB entry 8sxv coloured by chain, side view.
PDB entry 8sxv coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Structural analysis of a bacterial UDP-sugar 2-epimerase reveals the active site architecture before and after catalysis.
Thoden JB, McKnight JO, Kroft CW, Jast JDT, Holden HM
OpenAccess logo J Biol Chem 105200 (2023)
PMID: 37660908

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D-mannosamine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-228857 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine 2-epimerase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 367 amino acids
Theoretical weight: 41.12 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q72KY0 (Residues: 1-365; Coverage: 100%)
Gene name: TT_C0285
Sequence domains: UDP-N-acetylglucosamine 2-epimerase

Ligands and Environments

1 bound ligand:
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER D8 QUEST
Spacegroup: P21
Unit cell:
a: 61.675Å b: 88.926Å c: 87.567Å
α: 90° β: 98.79° γ: 90°
R-values:
R R work R free
0.202 0.198 0.259
Expression system: Escherichia coli BL21(DE3)

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