› 8sxw

X-ray diffraction
1.8Å resolution

X-ray crystal structure of UDP- 2,3-diacetamido-2,3-dideoxy-glucuronic acid-2-epimerase from Thermus thermophilus strain HB27, D98N mutation, apo structure at pH 6

Released:
DOI: 10.2210/pdb8sxw/pdb
PDB entry 8sxw coloured by chain, front view.
PDB entry 8sxw coloured by chain, side view.
PDB entry 8sxw coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Structural analysis of a bacterial UDP-sugar 2-epimerase reveals the active site architecture before and after catalysis.
Thoden JB, McKnight JO, Kroft CW, Jast JDT, Holden HM
OpenAccess logo J Biol Chem 105200 (2023)
PMID: 37660908

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D-mannosamine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-228857 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine 2-epimerase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 367 amino acids
Theoretical weight: 41.12 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q72KY0 (Residues: 1-365; Coverage: 100%)
Gene name: TT_C0285
Sequence domains: UDP-N-acetylglucosamine 2-epimerase

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER D8 QUEST
Spacegroup: P1
Unit cell:
a: 64.327Å b: 85.34Å c: 86.535Å
α: 108.23° β: 110.06° γ: 97.1°
R-values:
R R work R free
0.181 0.178 0.224
Expression system: Escherichia coli BL21(DE3)

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