› 8syh

X-ray diffraction
2Å resolution

X-ray crystal structure of UDP-2,3-diacetamido-2,3-dideoxy-glucuronic acid-2-epimerase from Thermus thermophilus strain HB27, D98N variant in the presence of UDP-2,3-diacetamido-2,3-dideoxy-glucuronic acid and UDP at pH 8

Released:
DOI: 10.2210/pdb8syh/pdb
PDB entry 8syh coloured by chain, front view.
PDB entry 8syh coloured by chain, side view.
PDB entry 8syh coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Structural analysis of a bacterial UDP-sugar 2-epimerase reveals the active site architecture before and after catalysis.
Thoden JB, McKnight JO, Kroft CW, Jast JDT, Holden HM
OpenAccess logo J Biol Chem 105200 (2023)
PMID: 37660908

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D-mannosamine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-565058 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine 2-epimerase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 373 amino acids
Theoretical weight: 41.99 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q72KY0 (Residues: 1-365; Coverage: 100%)
Gene name: TT_C0285
Sequence domains: UDP-N-acetylglucosamine 2-epimerase

Ligands and Environments

3 bound ligands:
Ligand MJL 2 x MJL
Ligand UDP 2 x UDP
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER D8 QUEST
Spacegroup: P21
Unit cell:
a: 56.788Å b: 128.158Å c: 58.475Å
α: 90° β: 112.54° γ: 90°
R-values:
R R work R free
0.186 0.183 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links