› 8szj

Electron Microscopy
3.35Å resolution

Human glutaminase C (Y466W) with L-Gln and Pi, filamentous form

Released:
DOI: 10.2210/pdb8szj/pdb
PDB entry 8szj coloured by chain, front view.
PDB entry 8szj coloured by chain, side view.
PDB entry 8szj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Filament formation drives catalysis by glutaminase enzymes important in cancer progression.
Feng S, Aplin C, Nguyen TT, Milano SK, Cerione RA
OpenAccess logo Nat Commun 15 1971 (2024)
PMID: 38438397
Related structures: EMD-40918

Function and Biology Details

Reaction catalysed: 
L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-271908 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaminase kidney isoform, mitochondrial 65 kDa chain Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 598 amino acids
Theoretical weight: 65.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O94925 (Residues: 1-550; Coverage: 82%)
  • Best match: O94925-3 (Residues: 1-598)
Gene names: GLS, GLS1, KIAA0838
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand PO4 12 x PO4
Ligand GLN 12 x GLN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.35Å
Relevant EMDB volumes: EMD-40918
Expression system: Escherichia coli

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