8t13

Electron Microscopy
3.45Å resolution

Cryo-EM structure of DENV2 NS5 in complex with human STAT2 with the N-terminal domain of STAT2 disordered

Released:

Function and Biology Details

Reactions catalysed:
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-273866 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Signal transducer and activator of transcription 2 Chain: A
Molecule details ›
Chain: A
Length: 851 amino acids
Theoretical weight: 98.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52630 (Residues: 1-851; Coverage: 100%)
Gene name: STAT2
Sequence domains:
Non-structural protein 5 Chain: B
Molecule details ›
Chain: B
Length: 900 amino acids
Theoretical weight: 103.26 KDa
Source organism: dengue virus type 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q91H74 (Residues: 2492-3391; Coverage: 27%)
Sequence domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.45Å
Relevant EMDB volumes: EMD-40953
Expression system: Escherichia coli BL21(DE3)