› 8t1j

Electron Microscopy
2.7Å resolution

Uncrosslinked nNOS-CaM oxygenase homodimer

Released:
DOI: 10.2210/pdb8t1j/pdb
PDB entry 8t1j coloured by chain, front view.
PDB entry 8t1j coloured by chain, side view.
PDB entry 8t1j coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Mapping interactions of calmodulin and neuronal NO synthase by crosslinking and mass spectrometry.
Felker D, Lee K, Pospiech TH, Morishima Y, Zhang H, Lau M, Southworth D, Osawa Y
OpenAccess logo J Biol Chem 105464 (2023)
PMID: 37979917
Related structures: EMD-40969

Function and Biology Details

Reaction catalysed: 
(1a) 2 L-arginine + 2 NADPH + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 NADP(+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151520 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitric oxide synthase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1429 amino acids
Theoretical weight: 160.77 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P29476 (Residues: 1-1429; Coverage: 100%)
Gene names: Bnos, Nos1
Sequence domains:

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
3 bound ligands:
Ligand ZN 1 x ZN
Ligand ARG 2 x ARG
Ligand H4B 2 x H4B
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-40969
Expression system: Escherichia coli

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Citations

1 mention without citation

Mapping interactions of calmodulin and neuronal NO synthase by crosslinking and mass spectrometry.
Felker et al. (2023)