› 8t1o

Electron Microscopy
3.3Å resolution

AP2 bound to MSP2N2 nanodisc with Tgn38 cargo peptide; composite map

Released:
DOI: 10.2210/pdb8t1o/pdb
PDB entry 8t1o coloured by chain, front view.
PDB entry 8t1o coloured by chain, side view.
PDB entry 8t1o coloured by chain, top view.
Source organisms:
Primary publication:
Lipid nanodiscs as a template for high-resolution cryo-EM structures of peripheral membrane proteins.
S Cannon K, Sarsam RD, Tedamrongwanish T, Zhang K, Baker RW
J Struct Biol 107989 (2023)
PMID: 37364761
Related structures: EMD-40973

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-221217 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
AP-2 complex subunit alpha-2 Chain: A
Molecule details ›
Chain: A
Length: 630 amino acids
Theoretical weight: 70.57 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P17427 (Residues: 1-271, 272-620; Coverage: 66%)
Gene names: Adtab, Ap2a2
Sequence domains: Adaptin N terminal region
AP-2 complex subunit beta Chain: B
Molecule details ›
Chain: B
Length: 591 amino acids
Theoretical weight: 66.95 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9DBG3 (Residues: 1-591; Coverage: 63%)
Gene names: Ap2b1, Clapb1
Sequence domains: Adaptin N terminal region
AP-2 complex subunit mu Chain: M
Molecule details ›
Chain: M
Length: 435 amino acids
Theoretical weight: 49.73 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P84091 (Residues: 1-435; Coverage: 100%)
Gene names: Ap2m1, Clapm1
Sequence domains:
AP-2 complex subunit sigma Chain: S
Molecule details ›
Chain: S
Length: 142 amino acids
Theoretical weight: 17.04 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P62743 (Residues: 1-142; Coverage: 100%)
Gene names: Ap17, Ap2s1, Claps2
Sequence domains: Clathrin adaptor complex small chain
Trans-Golgi network integral membrane protein TGN38 Chain: P
Molecule details ›
Chain: P
Length: 14 amino acids
Theoretical weight: 1.72 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19814 (Residues: 340-353; Coverage: 4%)
Gene name: Ttgn1

Ligands and Environments

1 bound ligand:
Ligand PIO 1 x PIO
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-40973
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Multi-monoubiquitylation controls VASP-mediated actin dynamics.
McCormick et al. (2024)