› 8t1p

Electron Microscopy
2.96Å resolution

Heterodimeric ABC transporter BmrCD in the occluded conformation bound to ADPVi: BmrCD_OC-ADPVi

Released:
DOI: 10.2210/pdb8t1p/pdb
PDB entry 8t1p coloured by chain, front view.
PDB entry 8t1p coloured by chain, side view.
PDB entry 8t1p coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Asymmetric conformations and lipid interactions shape the ATP-coupled cycle of a heterodimeric ABC transporter.
Tang Q, Sinclair M, Hasdemir HS, Stein RA, Karakas E, Tajkhorshid E, Mchaourab HS
Nat Commun 14 7184 (2023)
PMID: 37938578
Related structures: EMD-40974

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126595 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Probable multidrug resistance ABC transporter ATP-binding/permease protein YheI Chain: C
Molecule details ›
Chain: C
Length: 607 amino acids
Theoretical weight: 67.59 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: O07550 (Residues: 2-585; Coverage: 100%)
Gene names: BSU09710, yheI
Sequence domains:
Probable multidrug resistance ABC transporter ATP-binding/permease protein YheH Chain: D
Molecule details ›
Chain: D
Length: 681 amino acids
Theoretical weight: 77.47 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: O07549 (Residues: 1-673; Coverage: 100%)
Gene names: BSU09720, yheH
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand ATP 1 x ATP
Ligand MG 2 x MG
Ligand POV 21 x POV
Ligand AOV 1 x AOV
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.96Å
Relevant EMDB volumes: EMD-40974
Expression system: Escherichia coli

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