› 8t2j

X-ray diffraction
1.8Å resolution

Structure of the catalytic domain of PPM1D/Wip1 serine/threonine phosphatase

Released:
DOI: 10.2210/pdb8t2j/pdb
PDB entry 8t2j coloured by chain, front view.
PDB entry 8t2j coloured by chain, side view.
PDB entry 8t2j coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Kumar JP, Kosek D, Dyda F

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-245706 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein phosphatase 1D Chain: A
Molecule details ›
Chain: A
Length: 345 amino acids
Theoretical weight: 38.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15297 (Residues: 1-403; Coverage: 57%)
Gene names: PPM1D, WIP1
Sequence domains: Protein phosphatase 2C

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand PEG 1 x PEG
Ligand GOL 2 x GOL
1 modified residue:
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 49.664Å b: 59.93Å c: 49.566Å
α: 90° β: 92.13° γ: 90°
R-values:
R R work R free
0.176 0.175 0.219
Expression system: Escherichia coli BL21(DE3)

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