› 8t33

X-ray diffraction
1.6Å resolution

Crystal structure of K46 acetylated GABARAP in complex with the LIR of TP53INP2/DOR

Released:
DOI: 10.2210/pdb8t33/pdb
PDB entry 8t33 coloured by chain, front view.
PDB entry 8t33 coloured by chain, side view.
PDB entry 8t33 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional characterization of the role of acetylation on the interactions of the human Atg8-family proteins with the autophagy receptor TP53INP2/DOR.
Ali MG, Wahba HM, Igelmann S, Cyr N, Ferbeyre G, Omichinski JG
Autophagy (2024)
PMID: 38726830

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-271918 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Gamma-aminobutyric acid receptor-associated protein Chain: A
Molecule details ›
Chain: A
Length: 119 amino acids
Theoretical weight: 14.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95166 (Residues: 1-117; Coverage: 100%)
Gene names: FLC3B, GABARAP, HT004
Sequence domains: Autophagy protein Atg8 ubiquitin like
Tumor protein p53-inducible nuclear protein 2 Chain: B
Molecule details ›
Chain: B
Length: 15 amino acids
Theoretical weight: 1.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8IXH6 (Residues: 31-43; Coverage: 6%)
Gene names: C20orf110, DOR, PINH, TP53INP2

Ligands and Environments

2 bound ligands:
Ligand ZN 9 x ZN
Ligand ACT 4 x ACT
1 modified residue:
Ligand ALY 1 x ALY

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: C2221
Unit cell:
a: 53.488Å b: 89.178Å c: 63.967Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.212
Expression system: Escherichia coli BL21(DE3)

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