› 8t5s

Electron Microscopy
2.9Å resolution

Cryo-EM structure of DRH-1 helicase and C-terminal domain bound to dsRNA

Released:
DOI: 10.2210/pdb8t5s/pdb
PDB entry 8t5s coloured by chain, front view.
PDB entry 8t5s coloured by chain, side view.
PDB entry 8t5s coloured by chain, top view.
Source organisms:
Primary publication:
Caenorhabditis elegans Dicer acts with the RIG-I-like helicase DRH-1 and RDE-4 to cleave dsRNA.
Consalvo CD, Aderounmu AM, Donelick HM, Aruscavage PJ, Eckert DM, Shen PS, Bass BL
OpenAccess logo Elife 13 (2024)
PMID: 38747717
Related structures: EMD-41060

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-244365 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Dicer-related helicase Chain: A
Molecule details ›
Chain: A
Length: 1091 amino acids
Theoretical weight: 125.16 KDa
Source organism: Caenorhabditis elegans
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: G5EDI8 (Residues: 1-1037; Coverage: 100%)
Gene names: CELE_F15B10.2, F15B10.2, drh-1
Sequence domains:
RNA (30-MER) Chain: C
Molecule details ›
Chain: C
Length: 30 nucleotides
Theoretical weight: 9.14 KDa
Source organism: synthetic construct
Expression system: Not provided
RNA (30-MER) Chain: B
Molecule details ›
Chain: B
Length: 30 nucleotides
Theoretical weight: 9.83 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand MG 1 x MG
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-41060
Expression systems:
  • Spodoptera frugiperda
  • Not provided

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