› 8t5v

X-ray diffraction
1.79Å resolution

Influenza PA-N Endonuclease A36V mutant with Baloxavir

Released:
DOI: 10.2210/pdb8t5v/pdb
PDB entry 8t5v coloured by chain, front view.
PDB entry 8t5v coloured by chain, side view.
PDB entry 8t5v coloured by chain, top view.
Source organism: Influenza A virus (A/California/04/2009(H1N1))
Primary publication:
Structural Studies of Inhibitors with Clinically Relevant Influenza Endonuclease Variants.
Kohlbrand AJ, Stokes RW, Sankaran B, Cohen SM
OpenAccess logo Biochemistry 63 264-272 (2024)
PMID: 38190441

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-503966 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase acidic protein Chain: A
Molecule details ›
Chain: A
Length: 192 amino acids
Theoretical weight: 22.45 KDa
Source organism: Influenza A virus (A/California/04/2009(H1N1))
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C3W5S0 (Residues: 1-198; Coverage: 26%)
Gene name: PA

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand E4Z 1 x E4Z
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P6222
Unit cell:
a: 74.724Å b: 74.724Å c: 121.619Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.232 0.284
Expression system: Escherichia coli BL21(DE3)

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