› 8t77

Electron Microscopy
3.25Å resolution

SpRY-Cas9:gRNA complex bound to non-target DNA with 6 bp R-loop

Released:
DOI: 10.2210/pdb8t77/pdb
PDB entry 8t77 coloured by chain, front view.
PDB entry 8t77 coloured by chain, side view.
PDB entry 8t77 coloured by chain, top view.
Source organisms:
Primary publication:
Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9.
Hibshman GN, Bravo JPK, Hooper MM, Dangerfield TL, Zhang H, Finkelstein IJ, Johnson KA, Taylor DW
Nat Commun 15 3663 (2024)
PMID: 38688943
Related structures: EMD-41086

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-243679 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: A
Molecule details ›
Chain: A
Length: 1369 amino acids
Theoretical weight: 159.15 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99ZW2 (Residues: 2-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
TS Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Escherichia phage Lambda
Expression system: Not provided
NTS Chain: D
Molecule details ›
Chain: D
Length: 9 nucleotides
Theoretical weight: 2.73 KDa
Source organism: Escherichia phage Lambda
Expression system: Not provided
gRNA Chain: B
Molecule details ›
Chain: B
Length: 89 nucleotides
Theoretical weight: 28.72 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.25Å
Relevant EMDB volumes: EMD-41086
Expression systems:
  • Escherichia coli
  • Not provided

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