› 8t7h

Electron Microscopy
3.3Å resolution

Quis-bound intermediate mGlu5

Released:
DOI: 10.2210/pdb8t7h/pdb
PDB entry 8t7h coloured by chain, front view.
PDB entry 8t7h coloured by chain, side view.
PDB entry 8t7h coloured by chain, top view.
Source organisms:
Primary publication:
Stepwise activation of a metabotropic glutamate receptor.
Krishna Kumar K, Wang H, Habrian C, Latorraca NR, Xu J, O'Brien ES, Zhang C, Montabana E, Koehl A, Marqusee S, Isacoff EY, Kobilka BK
Nature (2024)
PMID: 38632403
Related structures: EMD-41092

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-273721 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Metabotropic glutamate receptor 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 881 amino acids
Theoretical weight: 98.87 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P41594 (Residues: 20-876; Coverage: 72%)
Gene names: GPRC1E, GRM5, MGLUR5
Sequence domains:
Nanobody 43 Chains: C, D
Molecule details ›
Chains: C, D
Length: 123 amino acids
Theoretical weight: 13.35 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand QUS 2 x QUS
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-41092
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Delineating the stepwise millisecond allosteric activation mechanism of the class C GPCR dimer mGlu5.
Li et al. (2024)

1 mention without citation

Step-wise activation of a Family C GPCR.
Kumar et al. (2023)