› 8t7t

Electron Microscopy
3.2Å resolution

CryoEM structure of the HisRS-like domain of human GCN2

Released:
DOI: 10.2210/pdb8t7t/pdb
PDB entry 8t7t coloured by chain, front view.
PDB entry 8t7t coloured by chain, side view.
PDB entry 8t7t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The HisRS-like domain of GCN2 is a pseudoenzyme that can bind uncharged tRNA.
Yin JZ, Keszei AFA, Houliston S, Filandr F, Beenstock J, Daou S, Kitaygorodsky J, Schriemer DC, Mazhab-Jafari MT, Gingras AC, Sicheri F
Structure 32 795-811.e6 (2024)
PMID: 38531363
Related structures: EMD-41094

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192842 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
eIF-2-alpha kinase GCN2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1650 amino acids
Theoretical weight: 187.22 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9P2K8 (Residues: 1-1649; Coverage: 100%)
Gene names: EIF2AK4, GCN2, KIAA1338
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-41094
Expression system: Spodoptera frugiperda

Quick links

Citations

1 citations in other articles

Gcn2 structurally mimics and functionally repurposes the HisRS enzyme for the integrated stress response.
Bou-Nader et al. (2024)