8t9g

Electron Microscopy
6.2Å resolution

Function and Biology Details

Reaction catalysed:
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(27) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(27)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 21-mer (preferred)
PDBe Complex ID:
PDB-CPX-275844 (preferred)
Entry contents:
10 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (12 distinct):
Polycomb protein SUZ12 Chains: B, G
Molecule details ›
Chains: B, G
Length: 619 amino acids
Theoretical weight: 72.27 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
Histone-lysine N-methyltransferase EZH2 Chains: C, I
Molecule details ›
Chains: C, I
Length: 753 amino acids
Theoretical weight: 86.17 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
Polycomb protein EED Chains: F, K
Histone-binding protein RBBP4 Chains: L, O
Zinc finger protein AEBP2 Chains: M, Y
Histone H3.2 Chains: A, W
Histone H4 Chains: J, X
Histone H2A type 1 Chains: R, U
Histone H2B 1.1 Chains: S, V
activating methylated peptide Chain: E
DNA (226-MER) Chain: H
DNA (226-MER) Chain: T

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 6.2Å
Relevant EMDB volumes: EMD-41110
Expression systems:
  • Trichoplusia ni
  • Escherichia coli
  • Not provided