› 8tah

Electron Microscopy
2.89Å resolution

Cryo-EM structure of Cortactin-bound to Arp2/3 complex

Released:
DOI: 10.2210/pdb8tah/pdb
PDB entry 8tah coloured by chain, front view.
PDB entry 8tah coloured by chain, side view.
PDB entry 8tah coloured by chain, top view.
Source organisms:
Primary publication:
Mechanism of synergistic activation of Arp2/3 complex by cortactin and WASP-family proteins.
Fregoso FE, Boczkowska M, Rebowski G, Carman PJ, van Eeuwen T, Dominguez R
OpenAccess logo Nat Commun 14 6894 (2023)
PMID: 37898612
Related structures: EMD-41135

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-229676 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Actin-related protein 3 Chain: A
Molecule details ›
Chain: A
Length: 418 amino acids
Theoretical weight: 47.43 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P61157 (Residues: 1-418; Coverage: 100%)
Gene names: ACTR3, ARP3
Sequence domains: Actin
Actin-related protein 2 Chain: B
Molecule details ›
Chain: B
Length: 394 amino acids
Theoretical weight: 44.82 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: A7MB62 (Residues: 1-394; Coverage: 100%)
Gene names: ACTR2, ARP2
Sequence domains: Actin
Actin-related protein 2/3 complex subunit 1A Chain: C
Molecule details ›
Chain: C
Length: 370 amino acids
Theoretical weight: 41.59 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q1JP79 (Residues: 1-370; Coverage: 100%)
Gene name: ARPC1A
Sequence domains: WD domain, G-beta repeat
Actin-related protein 2/3 complex subunit 2 Chain: D
Molecule details ›
Chain: D
Length: 300 amino acids
Theoretical weight: 34.4 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3MHR7 (Residues: 1-300; Coverage: 100%)
Gene name: ARPC2
Sequence domains: Arp2/3 complex, 34 kD subunit p34-Arc
Actin-related protein 2/3 complex subunit 3 Chain: E
Molecule details ›
Chain: E
Length: 178 amino acids
Theoretical weight: 20.57 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3T035 (Residues: 1-178; Coverage: 100%)
Gene name: ARPC3
Sequence domains: ARP2/3 complex ARPC3 (21 kDa) subunit
Actin-related protein 2/3 complex subunit 4 Chain: F
Molecule details ›
Chain: F
Length: 168 amino acids
Theoretical weight: 19.7 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q148J6 (Residues: 1-168; Coverage: 100%)
Gene name: ARPC4
Sequence domains: ARP2/3 complex 20 kDa subunit (ARPC4)
Actin-related protein 2/3 complex subunit 5 Chain: G
Molecule details ›
Chain: G
Length: 151 amino acids
Theoretical weight: 16.31 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: G3MXC8 (Residues: 1-48; Coverage: 32%)
Gene name: ARPC5
Src substrate cortactin Chain: H
Molecule details ›
Chain: H
Length: 76 amino acids
Theoretical weight: 8.68 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q60598 (Residues: 1-76; Coverage: 14%)
Gene names: Cttn, Ems1

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.89Å
Relevant EMDB volumes: EMD-41135
Expression system: Escherichia coli

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