› 8tbn

X-ray diffraction
1.46Å resolution

Function and Biology Details

Reactions catalysed: 
GTP + H(2)O = GDP + phosphate
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-524737 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTPase KRas, N-terminally processed Chains: A, B
Molecule details ›
Chains: A, B
Length: 170 amino acids
Theoretical weight: 19.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01116 (Residues: 1-169; Coverage: 89%)
  • Best match: P01116-2 (Residues: 1-169)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family
Peptidyl-prolyl cis-trans isomerase A Chains: C, D
Molecule details ›
Chains: C, D
Length: 166 amino acids
Theoretical weight: 18.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62937 (Residues: 1-165; Coverage: 100%)
Gene names: CYPA, PPIA
Sequence domains: Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD

Ligands and Environments

3 bound ligands:
Ligand GNP 2 x GNP
Ligand MG 2 x MG
Ligand ZNI 2 x ZNI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-2
Spacegroup: P212121
Unit cell:
a: 65.776Å b: 84.343Å c: 126.632Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.153 0.197
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Right ON target: a new RAS-GTP inhibitor.
Senft D. (2024)

1 mention without citation

Concurrent inhibition of oncogenic and wild-type RAS-GTP for cancer therapy.
Holderfield et al. (2024)