› 8tc3

Electron Microscopy
2.57Å resolution

CryoEM structure of nucleotide-free form of the nitrogenase iron protein from A. vinelandii

Released:
DOI: 10.2210/pdb8tc3/pdb
PDB entry 8tc3 coloured by chain, front view.
PDB entry 8tc3 coloured by chain, side view.
PDB entry 8tc3 coloured by chain, top view.
Source organisms:
Primary publication:
Anaerobic cryoEM protocols for air-sensitive nitrogenase proteins.
Warmack RA, Wenke BB, Spatzal T, Rees DC
Nat Protoc (2024)
PMID: 38575747
Related structures: EMD-41151

Function and Biology Details

Reaction catalysed: 
8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-246312 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein plum; Nitrogenase iron protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 518 amino acids
Theoretical weight: 57.2 KDa
Source organisms: Expression system: unidentified
UniProt:
  • Canonical: P00459 (Residues: 1-290; Coverage: 100%)
  • Canonical: Q5S3G7 (Residues: 2-226; Coverage: 100%)
Gene name: nifH1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand SF4 1 x SF4
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.57Å
Relevant EMDB volumes: EMD-41151
Expression system: unidentified

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