› 8tcb

X-ray diffraction
2.69Å resolution

Structure of human C4b-binding protein alpha chain CCP domains 1 and 2 in complex with the hypervariable region of group A Streptococcus M87 protein

Released:
DOI: 10.2210/pdb8tcb/pdb
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PDB entry 8tcb coloured by chain, front view.
PDB entry 8tcb coloured by chain, side view.
PDB entry 8tcb coloured by chain, top view.
Source organisms:
Primary publication:
Conservation of C4BP-binding sequence patterns in Streptococcus pyogenes M and Enn proteins.
Kolesiński P, McGowan M, Botteaux A, Smeesters PR, Ghosh P
J Biol Chem 300 107478 (2024)
PMID: 38879009

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-283202 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
C4b-binding protein alpha chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 128 amino acids
Theoretical weight: 14.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04003 (Residues: 49-172; Coverage: 23%)
Gene names: C4BP, C4BPA
Sequence domains: Sushi repeat (SCR repeat)
Gram-positive cocci surface proteins LPxTG domain-containing protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 104 amino acids
Theoretical weight: 12.53 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: W0T311 (Residues: 42-141; Coverage: 28%)
Gene name: emm87

Ligands and Environments

1 bound ligand:
Ligand K 1 x K
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-E
Unit cell:
a: 43.176Å b: 73.802Å c: 190.398Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.235 0.278
Expression system: Escherichia coli

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