› 8tcs

X-ray diffraction
1.5Å resolution

Structure of trehalose bound Alistipes sp. 3-Keto-beta-glucopyranoside-1,2-Lyase AL1

Released:
DOI: 10.2210/pdb8tcs/pdb
PDB entry 8tcs coloured by chain, front view.
PDB entry 8tcs coloured by chain, side view.
PDB entry 8tcs coloured by chain, top view.
Source organism: Alistipes
Primary publication:
An alternative broad-specificity pathway for glycan breakdown in bacteria.
Nasseri SA, Lazarski AC, Lemmer IL, Zhang CY, Brencher E, Chen HM, Sim L, Panwar D, Betschart L, Worrall LJ, Brumer H, Strynadka NCJ, Withers SG
Nature (2024)
PMID: 38898276

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-247755 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Xylose isomerase-like TIM barrel domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 270 amino acids
Theoretical weight: 30.76 KDa
Source organism: Alistipes
Expression system: Escherichia coli

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand CO 4 x CO
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 47.994Å b: 113.549Å c: 212.442Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.205
Expression system: Escherichia coli

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