8tdr

X-ray diffraction
3.32Å resolution

Crystal structure of the methyltransferase domain of DNMT3A homotetramer

Released:
Model geometry
Fit model/data
Source organism: Homo sapiens

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
PDBe Complex ID:
PDB-CPX-275530 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA (cytosine-5)-methyltransferase 3A Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 287 amino acids
Theoretical weight: 32.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y6K1 (Residues: 628-912; Coverage: 31%)
Gene name: DNMT3A
Sequence domains: C-5 cytosine-specific DNA methylase

Ligands and Environments


Cofactor: Ligand SAH 4 x SAH
No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P3
Unit cell:
a: 179.429Å b: 179.429Å c: 108.065Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.21 0.24
Expression system: Escherichia coli BL21(DE3)