› 8tfk

Electron Microscopy
2.66Å resolution

Cryo-EM structure of the Methanosarcina mazei glutamine synthetase (GS) with Met-Sox-P and ADP

Released:
DOI: 10.2210/pdb8tfk/pdb
PDB entry 8tfk coloured by chain, front view.
PDB entry 8tfk coloured by chain, side view.
PDB entry 8tfk coloured by chain, top view.
Source organism: Methanosarcina mazei Go1
Primary publication:
M. mazei glutamine synthetase and glutamine synthetase-GlnK1 structures reveal enzyme regulation by oligomer modulation.
Schumacher MA, Salinas R, Travis BA, Singh RR, Lent N
OpenAccess logo Nat Commun 14 7375 (2023)
PMID: 37968329
Related structures: EMD-41232

Function and Biology Details

Reaction catalysed: 
ATP + L-glutamate + NH(3) = ADP + phosphate + L-glutamine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235952 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamine synthetase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 467 amino acids
Theoretical weight: 52.83 KDa
Source organism: Methanosarcina mazei Go1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8PY99 (Residues: 1-447; Coverage: 100%)
Gene names: MM_0964, glnA1
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand MG 36 x MG
Ligand P3S 12 x P3S
Ligand ADP 12 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.66Å
Relevant EMDB volumes: EMD-41232
Expression system: Escherichia coli

Quick links