› 8tfq

X-ray diffraction
1.8Å resolution

Fab from C10-S66K antibody in complex with fentanyl

Released:
DOI: 10.2210/pdb8tfq/pdb
PDB entry 8tfq coloured by chain, front view.
PDB entry 8tfq coloured by chain, side view.
PDB entry 8tfq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An Engineered Human-Antibody Fragment with Fentanyl Pan-Specificity That Reverses Carfentanil-Induced Respiratory Depression.
Eubanks LM, Pholcharee T, Oyen D, Natori Y, Zhou B, Wilson IA, Janda KD
ACS Chem Neurosci (2023)
PMID: 37534714

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-227001 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Light chain from Fab of C10_S66K antibody Chains: A, L
Molecule details ›
Chains: A, L
Length: 214 amino acids
Theoretical weight: 22.9 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Heavy chain from Fab of C10_S66K antibody Chains: B, H
Molecule details ›
Chains: B, H
Length: 223 amino acids
Theoretical weight: 23.83 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

1 bound ligand:
Ligand 7V7 2 x 7V7
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 71.288Å b: 73.354Å c: 95.197Å
α: 90° β: 90.53° γ: 90°
R-values:
R R work R free
0.18 0.178 0.216
Expression system: Homo sapiens

Quick links

Citations

1 citations in other articles

Investigation of monoclonal antibody CSX-1004 for fentanyl overdose.
Bremer et al. (2023)