› 8tfu

X-ray diffraction
1.48Å resolution

Structure of Red beta C-terminal domain in complex with SSB C-terminal peptide, Form 1

Released:
DOI: 10.2210/pdb8tfu/pdb
PDB entry 8tfu coloured by chain, front view.
PDB entry 8tfu coloured by chain, side view.
PDB entry 8tfu coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the interaction of RedĪ² single strand annealing protein with Escherichia coli single-stranded DNA binding protein.
Zakharova K, Liu M, Greenwald JR, Caldwell BC, Qi Z, Wysocki VH, Bell CE
J Mol Biol 168590 (2024)
PMID: 38663547

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-527898 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Recombination protein bet Chains: A, B
Molecule details ›
Chains: A, B
Length: 84 amino acids
Theoretical weight: 9.43 KDa
Source organism: Escherichia phage Lambda
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P03698 (Residues: 182-261; Coverage: 31%)
Gene names: bet, betA, red-beta, redB
Plasmid-derived single-stranded DNA-binding protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 10 amino acids
Theoretical weight: 1.3 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P28044 (Residues: 166-175; Coverage: 6%)
Gene name: ssb

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 37.81Å b: 68.252Å c: 70.888Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.182 0.22
Expression systems:
  • Escherichia coli BL21
  • Not provided

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