› 8tgo

X-ray diffraction
5.75Å resolution

Crystal structure of the BG505 triple tandem trimer gp140 HIV-1 Env in complex with PGT124 and 35O22

Released:
DOI: 10.2210/pdb8tgo/pdb
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PDB entry 8tgo coloured by chain, front view.
PDB entry 8tgo coloured by chain, side view.
PDB entry 8tgo coloured by chain, top view.
Source organisms:
Primary publication:
Triple tandem trimer immunogens for HIV-1 and influenza nucleic acid-based vaccines.
Del Moral-Sánchez I, Wee EG, Xian Y, Lee WH, Allen JD, Torrents de la Peña A, Fróes Rocha R, Ferguson J, León AN, Koekkoek S, Schermer EE, Burger JA, Kumar S, Zwolsman R, Brinkkemper M, Aartse A, Eggink D, Han J, Yuan M, Crispin M, Ozorowski G, Ward AB, Wilson IA, Hanke T, Sliepen K, Sanders RW
OpenAccess logo NPJ Vaccines 9 74 (2024)
PMID: 38582771

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-243200 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (10 distinct):
Transmembrane protein gp41 Chains: B, H, a
Molecule details ›
Chains: B, H, a
Length: 164 amino acids
Theoretical weight: 17.86 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S6 (Residues: 509-661; Coverage: 18%)
Gene name: env
Sequence domains: Retroviral envelope protein
35O22 scFv Chains: D, L, b
Molecule details ›
Chains: D, L, b
Length: 286 amino acids
Theoretical weight: 30.34 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
PGT124 light chain Chains: C, P, c
Envelope glycoprotein gp160 Chains: G, R, e
PGT124 heavy chain Chains: O, S, f

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand NAG 31 x NAG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL12-1
Spacegroup: C2
Unit cell:
a: 357.592Å b: 212.044Å c: 207.71Å
α: 90° β: 125.07° γ: 90°
R-values:
R R work R free
0.244 0.242 0.292
Expression system: Homo sapiens

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