› 8th6

X-ray diffraction
2.34Å resolution

Function and Biology Details

Reactions catalysed: 
ATP + H(2)O = ADP + phosphate
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-224519 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras GTPase-activating protein-binding protein 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 139 amino acids
Theoretical weight: 15.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13283 (Residues: 1-139; Coverage: 30%)
Gene names: G3BP, G3BP1
Sequence domains: Nuclear transport factor 2 (NTF2) domain
Ubiquitin carboxyl-terminal hydrolase 10 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 24 amino acids
Theoretical weight: 2.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14694 (Residues: 2-24; Coverage: 3%)
  • Best match: Q14694-2 (Residues: 50-72)
Gene names: KIAA0190, USP10

Ligands and Environments

1 bound ligand:
Ligand EDO 15 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P21
Unit cell:
a: 58.39Å b: 78.38Å c: 73.41Å
α: 90° β: 108.63° γ: 90°
R-values:
R R work R free
0.217 0.213 0.295
Expression system: Escherichia coli

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