› 8thi

Electron Microscopy
3.36Å resolution

Cryo-EM structure of the Tripartite ATP-independent Periplasmic (TRAP) transporter SiaQM from Haemophilus influenzae (parallel dimer)

Released:
DOI: 10.2210/pdb8thi/pdb
PDB entry 8thi coloured by chain, front view.
PDB entry 8thi coloured by chain, side view.
PDB entry 8thi coloured by chain, top view.
Source organism: Haemophilus influenzae Rd KW20
Primary publication:
Structural and biophysical analysis of a Haemophilus influenzae tripartite ATP-independent periplasmic (TRAP) transporter.
Currie MJ, Davies JS, Scalise M, Gulati A, Wright JD, Newton-Vesty MC, Abeysekera GS, Subramanian R, Wahlgren WY, Friemann R, Allison JR, Mace PD, Griffin MDW, Demeler B, Wakatsuki S, Drew D, Indiveri C, Dobson RCJ, North RA
Elife 12 (2024)
PMID: 38349818
Related structures: EMD-41265

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235829 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sialic acid TRAP transporter permease protein SiaT Chains: A, B
Molecule details ›
Chains: A, B
Length: 655 amino acids
Theoretical weight: 72.05 KDa
Source organism: Haemophilus influenzae Rd KW20
Expression system: Escherichia coli
UniProt:
  • Canonical: P44543 (Residues: 1-616; Coverage: 100%)
Gene names: HI_0147, siaQM, siaT
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand PGT 2 x PGT
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.36Å
Relevant EMDB volumes: EMD-41265
Expression system: Escherichia coli

Quick links