› 8thk

Electron Microscopy
2.6Å resolution

Cryo-EM structure of A61603-bound alpha-1A-adrenergic receptor in complex with heterotrimeric Gq-protein

Released:
DOI: 10.2210/pdb8thk/pdb
PDB entry 8thk coloured by chain, front view.
PDB entry 8thk coloured by chain, side view.
PDB entry 8thk coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of agonist specificity of α1A-adrenergic receptor.
Su M, Wang J, Xiang G, Do HN, Levitz J, Miao Y, Huang XY
OpenAccess logo Nat Commun 14 4819 (2023)
PMID: 37563160
Related structures: EMD-41267

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-271969 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-2; Guanine nucleotide-binding protein G(q) subunit alpha; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 28.08 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P04899 (Residues: 1-57, 182-182, 320-320, 348-349; Coverage: 17%)
  • Canonical: P63092 (Residues: 205-342, 344-345, 348-357, 359-379, 382-383, 386-386, 389-389; Coverage: 42%)
  • Canonical: P50148 (Residues: 311-311, 314-315, 345-346, 349-350, 355-359; Coverage: 3%)
Gene names: GAQ, GNAI2, GNAI2B, GNAQ, GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 358 amino acids
Theoretical weight: 39.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Single fab chain (scFv16) Chain: F
Molecule details ›
Chain: F
Length: 267 amino acids
Theoretical weight: 28.67 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Alpha-1A adrenergic receptor; Endolysin Chain: R
Molecule details ›
Chain: R
Length: 503 amino acids
Theoretical weight: 56.49 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00720 (Residues: 2-161; Coverage: 98%)
  • Canonical: P35348 (Residues: 15-350; Coverage: 66%)
Gene names: ADRA1A, ADRA1C, E
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CGZ 1 x CGZ
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.6Å
Relevant EMDB volumes: EMD-41267
Expression system: Spodoptera frugiperda

Quick links

Citations

1 review citation

Signalling of Adrenoceptors: Canonical Pathways and New Paradigms.
Mastos et al. (2024)
3 other articles

1 mention without citation

Computational Methods for the Discovery and Optimization of TAAR1 and TAAR5 Ligands.
Scarano et al. (2024)