› 8ti1

Electron Microscopy
2.9Å resolution

Cryo-EM structure of a SUR1/Kir6.2-Q52R ATP-sensitive potassium channel in the presence of PIP2 in the open conformation

Released:
DOI: 10.2210/pdb8ti1/pdb
PDB entry 8ti1 coloured by chain, front view.
PDB entry 8ti1 coloured by chain, side view.
PDB entry 8ti1 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of an open KATP channel reveals tandem PIP2 binding sites mediating the Kir6.2 and SUR1 regulatory interface.
Driggers CM, Kuo YY, Zhu P, ElSheikh A, Shyng SL
OpenAccess logo Nat Commun 15 2502 (2024)
PMID: 38509107
Related structures: EMD-41277

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-268977 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATP-sensitive inward rectifier potassium channel 11 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 390 amino acids
Theoretical weight: 43.69 KDa
Source organism: Rattus norvegicus
Expression system: Chlorocebus aethiops
UniProt:
  • Canonical: P70673 (Residues: 1-390; Coverage: 100%)
Gene name: Kcnj11
Sequence domains:
SUR1 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 1582 amino acids
Theoretical weight: 177.3 KDa
Source organism: Mesocricetus auratus
Expression system: Chlorocebus aethiops
UniProt:
  • Canonical: A0A1S4NYG1 (Residues: 1-1582; Coverage: 100%)
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand PT5 8 x PT5
Ligand K 4 x K
Ligand PEF 4 x PEF
Ligand P5S 8 x P5S
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-41277
Expression system: Chlorocebus aethiops

Quick links

Citations

2 citation in other articles

A loss-of-function mutation in KCNJ11 causing sulfonylurea-sensitive diabetes in early adult life.
Vedovato et al. (2024)
1 more

2 mentions without citation

Structure of an open KATP channel reveals tandem PIP2 binding sites mediating the Kir6.2 and SUR1 regulatory interface.
Driggers et al. (2024)
1 more