› 8ti4

X-ray diffraction
2.1Å resolution

monovalent bispecific IgG antibodies through novel electrostatic steering mutations at the CH1-CL interface

Released:
DOI: 10.2210/pdb8ti4/pdb
PDB entry 8ti4 coloured by chain, front view.
PDB entry 8ti4 coloured by chain, side view.
PDB entry 8ti4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Robust production of monovalent bispecific IgG antibodies through novel electrostatic steering mutations at the CH1-Cλ interface.
Bagert JD, Oganesyan V, Chiang CI, Iannotti M, Lin J, Yang C, Payne S, McMahon W, Edwards S, Dippel A, Hutchinson M, Huang F, Aleti V, Niu C, Qian C, Denham J, Ferreira S, Pradhan P, Penney M, Wang C, Liu W, Walseng E, Mazor Y
OpenAccess logo MAbs 15 2273449 (2023)
PMID: 37930310

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235976 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
IgG1 Fab heavy chain, mutated to promote correct pairing Chain: H
Molecule details ›
Chain: H
Length: 224 amino acids
Theoretical weight: 23.9 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
IgG1 Fab light chain, mutated for better pairing with cognate heavy chain Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 23.06 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P212121
Unit cell:
a: 71.269Å b: 78.815Å c: 80.925Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.201 0.251
Expression system: Cricetulus griseus

Quick links

Citations

1 mention without citation

Robust production of monovalent bispecific IgG antibodies through novel electrostatic steering mutations at the CH1-Cλ interface.
Bagert et al. (2023)